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Stacey Wetmore: Profile Photo

Stacey Wetmore

Lethbridge, Alberta

Position: Professor and Tier 1 Canada Research Chair

Organization: University of Lethbridge

I am a researcher in computational chemistry, with pioneering contributions to nucleic acid chemistry. Research in my lab uniquely uses the full spectrum of computational approaches and close collaborations with experimentalists to study how naturally occurring or environmentally-derived nucleic acid derivatives are processed in cells, and the design of synthetic analogues with novel applications in medicine (bioprobes/drugs) or nanotechnology (biomaterials). My team has explained observed reactivities and predicted whether molecules will exhibit sought-after traits. Our research has globally impacted the way researchers think about the function of critical biomolecules and aided synthetic nucleic acid-analogue design. After completing my PhD at Dalhousie University and an NSERC-funded postdoctoral fellowship in Australia, I accepted a faculty position at Mount Allison in 2001 and was recruited to Lethbridge as a Tier 2 Canada Research Chair in 2006. My work has resulted in >190 publications and 127 invited talks, secured >$8 million in funding, and led to my current Tier 1 Canada Research Chair appointment. I have served on and chair national and international research, policy, and editorial boards, scientific peer review panels, society committees, and conference organization committees. I am a passionate mentor to all trainees in my lab from high school students to postdoctoral fellows, who have achieved numerous successes under my supervision and in their future careers.

Areas of Expertise:

+ Computational Chemistry
+ DNA Damage, Repair, and Replication
+ RNA Modifications and Therapeutics

Language(s):

+ English


My Work

What I do:

My mission is to use computer calculations to understand how chemical modifications affect the biological functions of DNA and RNA and to design new modifications with far-reaching applications. Nucleic acids are the most basic molecules of life, being tasked with storing and transmitting genetic information in all living organisms. To enhance nucleic acid programmability and stability, and aid the formation of functional 3D shapes, the fundamental building blocks of DNA and RNA are commonly modified. Furthermore, the ease of synthesis of nucleic acids functionalized at any nucleobase, sugar, or phosphate site, as well as the ability of modifications to impact pairing, chemical stability, conformation, and interactions with proteins, has led to the development of a wealth of unique modifications with far-reaching applications from drugs and vaccines to nanomachines. Unfortunately, the lack of known structure–function relationships for a range of modified nucleic acids raises questions such as how modifications improve organism survival and how modifications can be used to their full potential in valued applications. Computational chemistry provides a valuable tool to gain insights necessary to understand the chemistry of modified nucleic acid building blocks. Indeed, calculations can serve as powerful predictors of experimental outcomes and clarify discrepancies between experimental hypotheses and results.

Ask me about:

Anything related to DNA and RNA modifications, including their chemistry, biology, and real-life applications. My team enjoys using modeling to help experimentalists interpret their observations and direct synthesis of new modifications with diverse applications.

Why me:

My team makes me stand out in my field! I have worked hard to establish a collegial environment in my research lab that includes diverse trainees from high school students to undergraduate and graduate students to postdoctoral fellows. Every team member works to support one another to learn and accomplish their goals, including performing the best modeling that we can for our systems of interest. The hard work of my team has allowed us to join forces with excellent experimental collaborators in Canada and around the world. These collaborations have allows us to answer pressing questions related to the roles of nucleic acid modifications in nature and the design of new molecules for diverse applications.


About Me

Sector: Academia (Post Secondary)

English proficiency: Read, Write, Speak

Pronouns: She/Her/Hers

Gender: Female

Demographic: European / White


Recent Publications

Title Year
Modular access to nucleobase GFP-surrogates: pH-responsive smart probes for ratiometric nucleic acid diagnostics2025
Thieno[3,2-b]thiophene for the Construction of Far-Red Molecular Rotor Hemicyanines as High-Affinity DNA Aptamer Fluorogenic Reporters2024
Mechanism of Nucleic Acid Phosphodiester Bond Cleavage by Human Endonuclease V: MD and QM/MM Calculations Reveal a Versatile Metal Dependence2024
Unlocking precision in aptamer engineering: a case study of the thrombin binding aptamer illustrates why modification size, quantity, and position matter2024
Neutral Adducts of Molybdenum Hexafluoride Structure and Bonding in MoF6(NC5H5) and MoF6(NC5H5)22024
Fluoride-Ion Donor Properties of AsF52024
Stable Interstrand Cross-Links Generated from the Repair of 1,N6-Ethenoadenine in DNA by α-Ketoglutarate/Fe(II)-Dependent Dioxygenase ALKBH22024
Breaking the glass beaker: celebrating women’s contributions to chemistry in Canada2024
Is Metal Stabilization of the Leaving Group Required or Can Lysine Facilitate Phosphodiester Bond Cleavage in Nucleic Acids? A Computational Study of EndoV2024
Elucidation of the catalytic mechanism of a single-metal dependent homing endonuclease using QM and QM/MM approaches: the case study of I-PpoI2024
A computational study of the structures and base-pairing properties of pyrrolizidine alkaloid-derived DNA adducts2023
Unlocking Pb2+ Sensing Potential in a DNA G-Quadruplex via Loop Modification with Fluorescent Chalcone Surrogates2023
Generation of an accurate CCSD(T)/CBS data set and assessment of DFT methods for the binding strengths of group I metal–nucleic acid complexes2023
Harnessing a 4-Formyl-Aniline Handle to Tune the Stability of a DNA Aptamer–Protein Complex via Fluorescent Surrogates2023
Effect of Guanine Adduct Size, Shape, and Linker Type on the Conformation of Adducted DNA: A DFT and Molecular Dynamics Study2023
Introduction to the WATOC Special Issue2023
Assessment of Density Functional Theory Methods for the Structural Prediction of Transition and Post-Transition Metal–Nucleic Acid Complexes2023
Distinctive Formation of a DNA–Protein Cross-Link during the Repair of DNA Oxidative Damage: Insights into Human Disease from MD Simulations and QM/MM Calculations2023
Interaction Modes Between SF4 and Ketones; Study of Intermediates in Deoxofluorination Reactions2023
A modular aldol approach for internal fluorescent molecular rotor chalcone surrogates for DNA biosensing applications2023
Theoretical and Computational Chemistry2023
The Impact of DFT Functional, Cluster Model Size, and Implicit Solvation on the Structural Description of Single-Metal-Mediated DNA Phosphodiester Bond Cleavage: The Case Study of APE12022
Effects of a Second Local DNA Damage Event on the Toxicity of the Human Carcinogen 4-Aminobiphenyl: A Molecular Dynamics Study of a Damaged DNA Structure2022
The metal dependence of single-metal mediated phosphodiester bond cleavage: a QM/MM study of a multifaceted human enzyme2022
Lewis Acid Behavior of MoF5 and MoOF4: Syntheses and Characterization of MoF5(NCCH3), MoF5(NC5H5)n, and MoOF4(NC5H5)n (n = 1, 2)2021